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Recent Patents on Biotechnology

Author(s): Jonatas Oliveira da Silva, Michelli dos Santos, Ana Amélia Maia Silva, André Vinícius Fernandes Ferreira, Laís Moreira Nogueira, Isadora Braga Gandra, Silvio José Elisei Carvalho Junior, Juliana Martins Machado, Ana Alice Maia Gonçalves, Rodolfo Cordeiro Giunchetti, Mariana Campos-da-Paz and Alexsandro Sobreira Galdino*

DOI: 10.2174/1872208316666220902094706

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A Biotechnological Review on Patents Applied to Rubella Diagnosis

Page: [332 - 345] Pages: 14

  • * (Excluding Mailing and Handling)

Abstract

Background: Rubella, caused by the Rubella virus (RV), is considered a mild self-limited illness. However, RV has teratogenic potential. Laboratory investigation plays an important role in both diagnosis and surveillance of the disease. The main methods for diagnosing Rubella are serological assays for the detection of specific IgM and molecular assays for detecting viral RNA. However, some laboratories perform IgG avidity testing, virus isolation and analysis of genetic sequence as tools to help Rubella eradication. The importance of the diagnosis of Rubella involves the appropriate treatment of the disease, because the Rubella clinical symptoms may be similar to those of other diseases, and the population monitoring to avoid new emergent cases. This study addresses different methods of diagnosing Rubella and contributes as a source of knowledge to assist health systems in controlling the disease.

Objective: The main objective of this study was to review the available patents regarding Rubella diagnosis published in intellectual property databases, and provides an overview of the technologies available for the diagnosis of Rubella.

Method: The search strategy was based on the keywords searched separately or together using a Boolean operator either in the patent title or abstract the time interval was restricted to patents filed or granted from January 2009 until February 2022. The database used was Google Patents.

Results: This study analyzed 24 patent documents regarding strategies for the diagnosis of Rubella. Of these, 15 patents disclose strategies for detecting Rubella antibodies, 7 patents the detection of Rubella virus nucleic acid, and 2 patents the production of antibodies applied in Rubella diagnosis.

Conclusion: Rubella is still a public health problem in some countries, mainly those in development, especially due to congenital Rubella syndrome, which can cause malformation or fetal death. However, its diagnosis is challenging, due to similarity of symptoms with other diseases, and for this reason, laboratory diagnosis is essential. Studies like this encourage researchers and governments to invest in research to continue the development of new products, using different areas of biotechnology, to solve society's problems, especially diseases that have an impact on global health, such as Rubella.

Keywords: Rubella, German measles, Rubella virus, diagnosis Rubella, Rubella antibodies, recombinant proteins.

Graphical Abstract

[1]
Frey TK. Molecular biology of Rubella virus. Adv Virus Res 1994; 44: 69-160.
[http://dx.doi.org/10.1016/S0065-3527(08)60328-0] [PMID: 7817880]
[2]
Toizumi M, Vo HM, Dang DA, Moriuchi H, Yoshida LM. Clinical manifestations of congenital Rubella syndrome: A review of our experience in Vietnam. Vaccine 2019; 37(1): 202-9.
[http://dx.doi.org/10.1016/j.vaccine.2018.11.046] [PMID: 30503658]
[3]
Mawson AR, Croft AM. Rubella virus infection, the congenital Rubella syndrome, and the link to autism. Int J Environ Res Public Health 2019; 16(19): 3543.
[http://dx.doi.org/10.3390/ijerph16193543] [PMID: 31546693]
[4]
WHO. Rubella. 2021. Available from: https://www. who.int/news-room/fact-sheets/detail/Rubella (Accessed on: July 5, 2021).
[5]
Hübschen JM, Bork SM, Brown KE, et al. Challenges of measles and Rubella laboratory diagnostic in the era of elimination. Clin Microbiol Infect 2017; 23(8): 511-5.
[http://dx.doi.org/10.1016/j.cmi.2017.04.009] [PMID: 28412379]
[6]
Best JM. Rubella. Semin Fetal Neonatal Med 2007; 12(3): 182-92.
[http://dx.doi.org/10.1016/j.siny.2007.01.017] [PMID: 17337363]
[7]
Taku NA, Ndze VN, Abernathy E, et al. Seroprevalence of Rubella virus antibodies among pregnant women in the center and south-west regions of cameroon. PLoS One 2019; 14(11): e0225594.
[http://dx.doi.org/10.1371/journal.pone.0225594] [PMID: 31751431]
[8]
Hawkins SFC, Guest PC. Multiplex analyses using real-time quantitative PCR. Methods Mol Biol 2017; 1546: 125-33.
[9]
Souza M, Machado J, da Silva J, Ramos L, Nogueira L, Ribeiro P. Rational design and evaluation of the recombinant multiepitope protein for serodiagnosis of Rubella. Curr Pharm Biotechnol 2022; 23(8): 1094-100.
[http://dx.doi.org/10.2174/1389201022666210907170921] [PMID: 34493182]
[10]
Terpe K. Overview of bacterial expression systems for heterologous protein production: From molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 2006; 72(2): 211-22.
[http://dx.doi.org/10.1007/s00253-006-0465-8] [PMID: 16791589]
[11]
Hong B, Jing C, Yihang G, Yufen J, Xia X, Ting Y. Recombinant Rubella virus protein and application. Patent CN101781360, 2012.
[12]
Krieger MA, Zanchin NIT, Eugênio DS. Recombinant protein, synthetic DNA sequence, expression vector, host cell, composition, Rubella diagnostic kit, use of at least one recombinant protein, and methods for producing a recombinant protein and for diagnosing Rubella. Patent BR1020180753398, 2018.
[13]
Yu T, Bao H, Wu L, Jiang H, Xu T, Li F. Recombinant Rubella virus E1 protein and uses thereof. CN101402960, 2012.
[14]
Chen T, Kong X, Wang J, Zhang X. Recombinant Rubella virus protein and uses thereof. CN101509002, 2011.
[15]
Felipe MSS, Torres FAG, Galdino AS, Santos JCD, De Sauza MQ. Recombinant multiepitope protein, its procurement process and its applications related to Rubella. BR102012002469, 2015.
[16]
Galdino AS, Machado JM, Nogueira LM, Campos da Paz M, Mariano RMS. Synthetic peptide, its method of obtaining and its applications related to rubeola. Patent BR1020180730649, 2021.
[17]
Meili R, Fei G, Dong C. Rubella virus recombinant protein and application of same. CN104829731, 2015.
[18]
Yang Z, Gao H, Ding Z, Liu Z, Wang T. A kind of fusion protein for detecting Detecting Rubella Virus antibodies in human sera and preparation method thereof. CN107098980, 2017.
[19]
Scholz C, Bollhagen R, Engel A, Faatz E, Schaarschmidt P, Upmeier B. Novel Rubella E1 envelope protein variants and their use in the detection of anti-Rubella antibodies. EP2222694, 2015.
[20]
Chen M, Zhu Z, Liu D, et al. Rubella epidemic caused by genotype 1E Rubella viruses in Beijing, China, in 2007–2011. Virol J 2013; 10(1): 122.
[http://dx.doi.org/10.1186/1743-422X-10-122] [PMID: 23596982]
[21]
Qi D, Wang X, Chen J. Rubella virus antibody test kit. CN204832208, 2015.
[22]
Wang R, Tao Z, Wang C, Zhao J, Liu W, Wu X. Rubella virus IgG antibody affinity detection kit. CN105548537, 2016.
[23]
Viswanathan R, George S, Murhekar MV, et al. Comparison of two commercial ELISA kits for detection of Rubella specific IgM in suspected congenital Rubella syndrome cases and Rubella IgG antibodies in a serosurvey of pregnant women. Diagn Microbiol Infect Dis 2019; 94(3): 243-7.
[http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.009] [PMID: 30782464]
[24]
Li X, Zhang Q, Hou P, et al. Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections. Int J Mol Med 2015; 36(5): 1319-26.
[http://dx.doi.org/10.3892/ijmm.2015.2333] [PMID: 26329478]
[25]
Wukui H, Xiaoyan Y, Xueming M, Yin L, Jing W, Yunping L. Joint-detecting test paper for Rubella virus IgM and IgG antibody. CN202153226, 2012.
[26]
Ming L. Test paper strip for detecting morbilli and Rubella virus IgM antibody colloidal gold, method for making same and applications. CN101363857, 2012.
[27]
Foubert A, Beloglazova NV, De Saeger S. Comparative study of colloidal gold and quantum dots as labels for multiplex screening tests for multi-mycotoxin detection. Anal Chim Acta 2017; 955: 48-57.
[http://dx.doi.org/10.1016/j.aca.2016.11.042] [PMID: 28088280]
[28]
Yu J, Su J, Zhang J, Wei X, Guo A. CdTe/CdS quantum dot-labeled fluorescent immunochromatography test strips for rapid detection of Escherichia coli O157:H7. RSC Advances 2017; 7(29): 17819-23.
[http://dx.doi.org/10.1039/C7RA00821J]
[29]
Wen D, Shen Y, Yu X. Method for detecting Rubella virus, quantum dot-labeled immunochromatographic test paper and preparation method thereof. CN103529211, 2015.
[30]
Li K, Li J, Tao Z, Wang J, Fu Q, Liu G. Immunochromatographic kit for quantitative detection of RV (Rubella virus) IgG (immunoglobulin G) antibody through quantum dots. CN105067584, 2015.
[31]
Kumar H, Singh VA, Isha M, Mehta S, Garg R, Shinu P. Line Immunoassay: A rapid test for screening torch complex in antenatal patients with bad obstetric history. Mymensingh Med J 2018; 27(3): 641-4.
[PMID: 30141457]
[32]
Khuroo MS, Khuroo NS, Khuroo MS. Accuracy of rapid point-of-care diagnostic tests for hepatitis b surface antigen—a systematic review and meta-analysis. J Clin Exp Hepatol 2014; 4(3): 226-40.
[http://dx.doi.org/10.1016/j.jceh.2014.07.008] [PMID: 25755565]
[33]
Makanjuola RO, Taylor-Robinson AW. Improving accuracy of malaria diagnosis in underserved rural and remote endemic areas of Sub-Saharan Africa: A call to develop multiplexing rapid diagnostic tests. Scientifica 2020; 2020: 1-7.
[http://dx.doi.org/10.1155/2020/3901409]
[34]
Zanga J, Mbanzulu MK, Kabasele AF, Ngatu NR, Wumba DR. Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women. BMC Infect Dis 2017; 17(1): 250.
[http://dx.doi.org/10.1186/s12879-017-2352-6] [PMID: 28381214]
[35]
Uchino K, Miyoshi T, Mori Y, et al. Comparison of virological and serological methods for laboratory confirmation of Rubella. J Clin Virol 2020; 123: 104257.
[http://dx.doi.org/10.1016/j.jcv.2019.104257] [PMID: 31927348]
[36]
Dai L, Ai Y, Deng Z. Method and kit for detecting RV RNA (Rubella Virus Ribose Nucleic Acid). CN103725799, 2015.
[37]
Weixiang S, Jianjun H, Dazhi W, Yi X. Rubella virus fluorescence quantitative polymerase chain reaction (PCR) kit and detection method thereof. CN102061341, 2011.
[38]
Li R, Wang G, Liu Z, Yang J, Zhang X. A kind of fluorescence PCR method and kit of specific detection Rubella virus nucleic acid. CN106929608, 2017.
[39]
Haozhi L. Rubella virus detection and diagnosis kit and application thereof. CN102851393, 2013.
[40]
Xu C, Lu Y, Feng Y, Wu H, Zhong S. RT-PCR (Reverse Transcription-Polymerase Chain Reaction) fluorescence detection kit for Rubella virus and detection method thereof. CN102952897, 2013.
[41]
Li R, Wang G, Liu Z, Yang J, Zhang X. The kit for detecting nucleic acid of a kind of rapid detection Measles virus/Rubella virus. CN103409554, 2015.
[42]
Qi J, Liu J, Chen H, Zhang B, Wang C, Chen Y. Polymerase chain reaction (PCR) method for identifying four pathogens in prenatal and postnatal care examination through single tube and kit thereof. CN104120195, 2016.
[43]
Pan J, Zhou H, Min D. Heterogenous conjugate and application thereof to RV (Rubella virus) assay. CN104650236, 2018.
[44]
Burioni R, Clementi M. Antibodies specific for Rubella Virus. US20100143376, 2010.
[45]
Sohrabi C, Alsafi Z, O’Neill N, et al. World Health Organization declares global emergency: A review of the 2019 novel coronavirus (COVID-19). Int J Surg 2020; 76: 71-6.
[http://dx.doi.org/10.1016/j.ijsu.2020.02.034] [PMID: 32112977]
[46]
Brazil M da S. Health Surveillance Guide: single volume. 2021. Available from: https://bvsms.saude.gov.br/bvs/publicacoes/guia_vigilancia_saude_3ed.pdf (Accessed on: December 11, 2021).
[47]
Brazil. Rubella. 2021. Available from: https://www. gov.br/saude/pt-br/assuntos/saude-de-a-a-z/r/rubeola (Accessed on: December 11, 2021).