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The Natural Products Journal

Author(s): Stanila Stoeva*, Maya Radeva–Ilieva, Iliya Zhelev and Kaloyan Georgiev

DOI: 10.2174/2210315512666220512212448

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A HPLC-UV Method for Analysis of Total Plant Extract and Catechin Fraction of Bancha Green Tea

Article ID: e120522204703 Pages: 8

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Abstract

Background: The pharmacological characterization of Bancha green tea requires a preliminary establishment of its phytochemical composition.

Objective: The aim of the present study was to develop a simple, sensitive, and environmentally friendly HPLC-UV procedure for the simultaneous quantification of caffeine, (+)-catechin, epigallocatechin gallate and gallic acid in different Bancha green tea isolates.

Methods: The chromatographic separation of the four phytochemicals was performed on a Hypersil GOLD aQ column with a mobile phase consisting of acetonitrile/acetic acid/bi-distilled water (8:1:91 v/v/v) in an isocratic mode of elution. The suitability of the proposed procedure was verified by achieving satisfying linearity and specificity.

Results: The method was also assessed as accurate and precise, with the obtained limits of quantification of caffeine, (+)-catechin, epigallocatechin gallate, and gallic acid of 0.01, 0.30, 0.50 and 0.03 μg/mL, respectively. As it was expected, the analytical procedure approbation showed the highest epigallocatechin gallate content (16.78 % ± 0.54) in the catechin fraction. On the other hand, the amount of caffeine and epigallocatechin gallate in the total plant extract was almost the same (4.19 % ± 0.14 and 4.12 % ± 0.44, respectively).

Conclusion: A reliable HPLC-UV method for phytochemical characterization of Bancha green tea extracts has been developed. The conducted quantitate assay could be used as a basis for subsequent pharmacological in vivo and in vitro studies of Bancha green tea.

Keywords: Bancha, Green tea, Caffeine, (+)-Catechin, Epigallocatechin gallate, Gallic acid, HPLC

Graphical Abstract

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