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Protein & Peptide Letters

Author(s): Shally Sharma and Desh Deepak Singh*

DOI: 10.2174/0929866529999220509122720

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Investigations on the Biological Activity of Allium sativum Agglutinin (ASA) Isolated from Garlic

Page: [555 - 566] Pages: 12

  • * (Excluding Mailing and Handling)

Abstract

Background: Garlic (Allium sativum) from the family Amaryllidaceae is widely used in culinary and is reported to have potential anticancer, anti-diabetic, antimicrobial, and cardioprotective activities. Allium sativum agglutinin (ASA) is a bulb-type lectin (BTL) domaincontaining lectin isolated from garlic and has been studied for its various biological functions. Previous studies have reported the anti-cancer effects of ASA on histiocytic lymphoma (U937), promyelocytic leukemia (HL60), and oral cancer (KB).

Methods: In this study, we have purified and characterized ASA and evaluated it for its anticancer effects on other cancer cell lines. MTT assay and FACS analysis was done to corroborate the anticancer findings against cervical (HeLa) and lung cancer (A549) cell lines.

Results: IC50 value of 37 μg/ml in HeLa and a weak activity (26.4 ± 1.9% cellular inhibition at 100μg/ml treatment) in A549 were found in the MTT assay. FACS analysis further corroborated these findings and showed the apoptotic effects of ASA in these cell lines.

Conclusion: Anticancer activity for members of bulb-type lectin (BTL) domain-containing lectins has been widely reported, and we hope that our study forms a basis for the development of ASA as a therapeutic agent.

Keywords: Allium sativum agglutinin (ASA), fluorescence spectroscopy, circular dichroism, flow cytometry, anti-cancer property, lectins.

Graphical Abstract

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[http://dx.doi.org/10.1007/BF02703701] [PMID: 15886457]