Abstract
Background: Orchids are widely used in floriculture as attractive cut flowers all
over the world. The current paper reviews factors affecting the in vitro flowering in orchid
species.
Methods: The phenomenon of in vitro flowering is advantageous as it shortens the breeding
cycle, which is prolonged in the in vivo grown orchid species of commercial value.
Certain genetic and biochemical mechanisms are involved at the time of onset of flowering
in orchid plants. In the present endeavour, efforts have been made to initiate in vitro flowering
in terrestrial and epiphytic orchid species. Various phenomenon involved during transition
of vegetative shoot apex into floral axis is also reviewed.
Results: In vitro flowering was induced in Dendrobium nobile and Zeuxine strateumatica
in Mitra medium supplemented with BAP at 0.5 in mgl-1.
Conclusion: In vitro flowering can be initiated by manipulating physical and chemical
stimulus in the nutrient regime. The study would be helpful in better understanding the
complex process of flowering in vitro as well as ex vitro.
Keywords:
Orchid, cytokinins, auxins, in vitro flowering, culture medium, priced ornamentals
Graphical Abstract
[1]
Goh CJ, Arditti J. Orchidaceae. In: Halevy AH, Ed. Handbook of flowering. Florida: CRC Press In: 1985; 1: pp. 309-6.
[2]
Goh CJ. Studies on flowering in orchids: A review and future directions. Proceedings of the Nagoya International Orchid Show (NIOC '92). March, 12-15, 1992. Nagoya, Japan. 1992; pp. 44-9.
[3]
Ziv M, Naor V. Flowering of geophytes in vitro. Propag Ornam Plants 2006; 6: 3-16.
[4]
Dressler RL. Phylogeny and classification of the orchid family. Portland, Oregon: Dioscorides Press 1993.
[5]
Lucksom SZ. The orchids of Sikkim and North-east Himalaya Gangtok. East Sikkim, Assam, India: Author Publishers and Distributors 2007.
[13]
Wang GY, Xu ZH, Chua NH. In vitro flower formation of Cymbidium ensifolium. 4th Asia Pacific Orchid Conference Program and Abstracts Registrant’, Hand Book. 84
[19]
Duan JX, Yazawa S. Proceedings of the Nagoya International Orchid Show 1990; 87-96.
[22]
Mitra GC, Prasad RN, Chowdhury AR. Inorganic salts and differentiation of protocorm in seed callus of an orchid and correlated changes in its free amino acid content. Indian J Exp Biol 1976; 14: 350-1.
[24]
Wang X. Tissue culture of Cymbidium: Plant and flower induction in vitro. Lindleyana 1988; 3: 184-9.
[25]
Chia TF, Arditti J, Segeren M, Hew CS. Review: in vitro flowering of orchids. Lindleyana 1999; 14: 60-76.
[26]
Zhu GB. Rapid propagation protocol in the culture of Cymbidium kanran makino and in vitro flowering 2006.
[28]
Jia YJ, Cao YL, Wang S, Tang L, Ying XU, Fang C. Studies of the culture of internode of flower branch of China orchid. J Sichuan Uni, (Nat Sci Edition) 2000; 37: 94-7.
[33]
Zhang Y, Zhao S, Liu D, Zhang Q, Cheng J. Flowering phenology and reproductive characteristics of Cypripedium macranthos (Orchidaceae) in China and their implication in conservation. Pak J Bot 2014; 46(4): 1303-8.
[51]
Sherif AN, Kumar TS, Rao MV. In vitro propagation and genetic stability assessment of an endangered terrestrial jewel orchid Anoectochilus elatus Lindl. Indian J Exp Biol 2017; 55(12): 853-63.