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Current Traditional Medicine

Author(s): Majid Pirestani, Hossein Sobati* and Ali Dalir Ghaffari

DOI: 10.2174/2215083808666220410192848

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Investigation of Antiparasitic Effects of Eisenia fetida Extract (Annelida, Lumbricidae) against Toxoplasma gondii: In vitro Study

Article ID: e100422203354 Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: Toxoplasmosis, which is caused by Toxoplasma gondii, is a widespread parasitic disease that affects approximately one-third of the world's population. Due to the significant side effects of anti-Toxoplasma drugs, it is necessary to acquire new drugs with low toxicity, high activity, cheaper and more effective.

Objective: Earthworm has long been used as a therapeutic source of medicine in China and other parts of the Far East. Therefore, the purpose of the present study was to investigate of antiparasitic effects of Eisenia fetida extract against T. gondii in the Vero cell line.

Materials and Methods: The effects of the extract on the parasite, Vero cell, and the parasitized cells were estimated by MTT assay in vitro. Apoptosis was also measured by flow cytometer in three groups of extract-treated parasites, extract-treated Vero cells, and extract-treated parasitic Vero cells, respectively.

Results: The inhibitory effect of E. fetida extract and sulfadiazine was more than 85% and 65% on the parasite, respectively. Intracellular multiplication of the parasite in Vero cells during the 3 hours test showed a significant decrease compared to the 1-hour test (p<0.05). Flow cytometric analysis showed that with increasing concentration of E. fetida, apoptosis increased significantly (p<0.05).

Conclusion: The results showed that E. fetida extract in vitro could have a significant antiparasitic effect against T. gondii.

Keywords: Toxoplasma gondii, Eisenia fetida, sulfadiazine, cytotoxic activity, antiparasitic effect, in vitro.

[1]
Robert-Gangneux F, Dardé M-L. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev 2012; 25(2): 264-96.
[http://dx.doi.org/10.1128/CMR.05013-11] [PMID: 22491772]
[2]
Yousefvand A, Mirhosseini SA, Ghorbani M, Mohammadzadeh T, Moghaddam MM, Mohammadyari S. Molecular and serological detec-tion and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers. J Verbraucherschutz Lebensmsicherh 2021; 16(2): 117-27.
[http://dx.doi.org/10.1007/s00003-020-01306-w] [PMID: 33424528]
[3]
Kodym P, Malý M, Beran O, et al. Incidence, immunological and clinical characteristics of reactivation of latent Toxoplasma gondii infec-tion in HIV-infected patients. Epidemiol Infect 2015; 143(3): 600-7.
[http://dx.doi.org/10.1017/S0950268814001253] [PMID: 24850323]
[4]
Pereira KS, Franco RM, Leal DA. Transmission of toxoplasmosis (Toxoplasma gondii) by foods. Adv Food Nutr Res 2010; 60: 1-19.
[http://dx.doi.org/10.1016/S1043-4526(10)60001-0] [PMID: 20691951]
[5]
Ghasemi FS, Rasti S, Piroozmand A, et al. Toxoplasmosis-associated abortion and stillbirth in Tehran, Iran. J Matern Fetal Neonatal Med 2016; 29(2): 248-51.
[http://dx.doi.org/10.3109/14767058.2014.996127] [PMID: 25564725]
[6]
Konstantinovic N, Guegan H, Stäjner T, Belaz S, Robert-Gangneux F. Treatment of toxoplasmosis: Current options and future perspec-tives. Food Waterborne Parasitol 2019; 15e00036
[http://dx.doi.org/10.1016/j.fawpar.2019.e00036] [PMID: 32095610]
[7]
Gaafar MR, Mady RF, Diab RG, Shalaby TI. Chitosan and silver nanoparticles: Promising anti-toxoplasma agents. Exp Parasitol 2014; 143: 30-8.
[http://dx.doi.org/10.1016/j.exppara.2014.05.005] [PMID: 24852215]
[8]
Derouin F, Jacqz-Aigrain E, Thulliez P, Couvreur J, Leport C. Cotrimoxazole for prenatal treatment of congenital toxoplasmosis? Parasitol Today 2000; 16(6): 254-6.
[http://dx.doi.org/10.1016/S0169-4758(00)01667-7] [PMID: 10827434]
[9]
Grujić J, Djurković-Djaković O, Nikolić A, Klun I, Bobić B. Effectiveness of spiramycin in murine models of acute and chronic toxo-plasmosis. Int J Antimicrob Agents 2005; 25(3): 226-30.
[http://dx.doi.org/10.1016/j.ijantimicag.2004.09.015] [PMID: 15737517]
[10]
Omar HM, Ibraheim ZZ, El-Shimy NA, Ali RS. Anti-inflammatory, antipyretic and antioxidant activities of the earthworms extract. J Biol Earth Sci 2012; 2(1): 10-7.
[11]
Cooper EL, Balamurugan M, Huang C-Y, et al. Earthworms dilong: Ancient, inexpensive, noncontroversial models may help clarify ap-proaches to integrated medicine emphasizing neuroimmune systems. Evid Based Compl Alter Med 2012; 2012164152
[http://dx.doi.org/10.1155/2012/164152]
[12]
Wang D, Ruan Z, Zhang R, Wang X, Wang R, Tang Z. Effect of earthworm on wound healing: A systematic review and meta-analysis Front Pharmacol 2710; 12: 691742
[13]
Bansal N, Gupta RK, Nehra V. Antibacterial effects of Eisenia fetida, earthworm extract against pathogenic bacteria isolated from Cyprinus carpio. Int J Curr Microbiol Appl Sci 2018; 7(04): 2329-40.
[http://dx.doi.org/10.20546/ijcmas.2018.704.267]
[14]
Zarina Z, Noor Hasyierah MS, Alina Rahayu M, Ahmad Anas N, Siti Nur Amirah I. Optimization of protein content in earthworm-based fish feed formulation for catfish (Clarius gariepinus). Sains Malays 2012; 41(9): 1071-7.
[15]
Chachain NF, Jamil HA. Therapeutic effect of earthworm powder on the pathogenesis of Entamoeba histolytica in vivo. Pakistan. J Biotechnol 2017; 14(4): 643-51.
[16]
Balamurugan M, Parthasarathi K, Cooper EL, Ranganathan LS. Anti-inflammatory and anti-pyretic activities of earthworm extract-Lampito mauritii (Kinberg). J Ethnopharmacol 2009; 121(2): 330-2.
[http://dx.doi.org/10.1016/j.jep.2008.10.021] [PMID: 19022370]
[17]
Ansari AA, Sitaram K. An investigation into the anti-microbial and anti-fungal properties of earthworm powder obtained from Eisenia fetida. Am J Food Technol 2011; 6(4): 329-35.
[http://dx.doi.org/10.3923/ajft.2011.329.335]
[18]
Cooper EL, Balamurugan M. Unearthing a source of medicinal molecules. Drug Discov Today 2010; 15(21-22): 966-72.
[http://dx.doi.org/10.1016/j.drudis.2010.09.004] [PMID: 20932936]
[19]
Hrženjak T, Popović M, Bozić T, Grdisa M, Kobrehel D, Tiška-Rudman L. Fibrinolytic and anticoagulative activities from the earthworm Eisenia foetida. Comp Biochem Physiol B Biochem Mol Biol 1998; 119(4): 825-32.
[http://dx.doi.org/10.1016/S0305-0491(98)00060-1] [PMID: 9787773]
[20]
Wang C, Sun Z, Liu Y, Zhang X, Xu G. A novel antimicrobial vermipeptide family from earthworm Eisenia fetida. Eur J Soil Biol 2007; 43: S127-34.
[http://dx.doi.org/10.1016/j.ejsobi.2007.08.048]
[21]
Furtado GC, Slowik M, Kleinman HK, Joiner KA. Laminin enhances binding of Toxoplasma gondii tachyzoites to J774 murine macro-phage cells. Infect Immun 1992; 60(6): 2337-42.
[http://dx.doi.org/10.1128/iai.60.6.2337-2342.1992] [PMID: 1534073]
[22]
Moghaddam MM, Barjini KA, Ramandi MF, Amani J. Investigation of the antibacterial activity of a short cationic peptide against multi-drug-resistant Klebsiella pneumoniae and Salmonella typhimurium strains and its cytotoxicity on eukaryotic cells. World J Microbiol Biotechnol 2014; 30(5): 1533-40.
[http://dx.doi.org/10.1007/s11274-013-1575-y] [PMID: 24323118]
[23]
Sani MRM, Moghaddam MM, Aghamollaei H, Hassanpour K, Taheri RA, Farnoosh G. Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major Asian Pac J Trop Med 2014; 7S1: 7S2-3.
[http://dx.doi.org/10.1016/S1995-7645(14)60205-4] [PMID: 25312194]
[24]
Taghipour-Sabzevar V, Sharifi T, Bagheri-Khoulenjani S, et al. Targeted delivery of a short antimicrobial peptide against CD44-overexpressing tumor cells using hyaluronic acid-coated chitosan nanoparticles: An in vitro study. J Nanopart Res 2020; 22(5): 1-16.
[http://dx.doi.org/10.1007/s11051-020-04838-2]
[25]
Ferreira-da-Silva MF, Pons AH, Tedesco RC, Barbosa HS. Viability and infectivity analysis of Toxoplasma gondii under axenic condi-tions. Sci Med (Porto Alegre) 2010; 20(1): 93-8.
[26]
Villena I, Aubert D, Leroux B, et al. Pyrimethamine-sulfadoxine treatment of congenital toxoplasmosis: Follow-up of 78 cases between 1980 and 1997. Scand J Infect Dis 1998; 30(3): 295-300.
[http://dx.doi.org/10.1080/00365549850160963] [PMID: 9790140]
[27]
Grdiša M, Gršić K, Grdiša M. Earthworms-role in soil fertility to the use in medicine and as a food. Invertebrate Surviv J 2013; 10(1): 38-45.
[28]
Devanthran K, Unyah Z, Majid RA, Abdullah WO. In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites. Trop J Pharm Res 2017; 16(11): 2667-73.
[http://dx.doi.org/10.4314/tjpr.v16i11.14]
[29]
Saadatnia G, Haj Ghani H, Khoo BY, Maimunah A, Rahmah N. Optimization of Toxoplasma gondii cultivation in VERO cell line. Trop Biomed 2010; 27(1): 125-30.
[PMID: 20562822]
[30]
Kathireswari P, Alakesan A, Abirami P, Sangeetha P. Antimicrobial activity of earthworm coelomic fluid against diseases causing microor-ganisms. Int J Curr Microbiol Appl Sci 2014; 3(8): 608-13.
[31]
Mathur A, Verma SK, Bhat R, et al. Antimicrobial activity of earthworm extracts. J Chem Pharm Res 2010; 2(4): 364-70.
[32]
Shobha S, Kale RD. In vitro studies on control of soil-borne plant pathogens by earthworm eudrilus eugeniae exudates. Appl Soil Ecol 2008; 45(4): 26-38.
[33]
Prakash J, Gupta SK, Kochupillai V, Singh N, Gupta YK, Joshi S. Chemopreventive activity of Withania somnifera in experimentally in-duced fibrosarcoma tumours in Swiss albino mice. Phytother Res 2001; 15(3): 240-4.
[http://dx.doi.org/10.1002/ptr.779] [PMID: 11351360]
[34]
Prakash M, Gunasekaran G. Antibacterial activity of the indigenous earthworms Lampito mauritii (Kinberg) and Perionyx excavatus (Perri-er). J Altern Complement Med 2011; 17(2): 167-70.
[http://dx.doi.org/10.1089/acm.2009.0720] [PMID: 21299431]
[35]
Li W, Li S, Zhong J, Zhu Z, Liu J, Wang W. A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen). Peptides 2011; 32(6): 1146-50.
[http://dx.doi.org/10.1016/j.peptides.2011.04.015] [PMID: 21539875]
[36]
Engelmann P, Kiss J, Csöngei V, Cooper EL, Németh P. Earthworm leukocytes kill HeLa, HEp-2, PC-12 and PA317 cells in vitro. J Biochem Biophys Methods 2004; 61(1-2): 215-27.
[http://dx.doi.org/10.1016/j.jbbm.2004.04.004] [PMID: 15560938]
[37]
Popović M, Grdiša M, Hrženjak TM. Glycolipoprotein G-90 obtained from the earthworm Eisenia foetida exerts antibacterial activity. Vet Arh 2005; 75(2): 119-28.
[38]
Liu Z, Wang J, Zhang J, Yu B, Niu B. An extract from the earthworm Eisenia fetida non-specifically inhibits the activity of influenza and adenoviruses. J Tradit Chin Med 2012; 32(4): 657-63.
[http://dx.doi.org/10.1016/S0254-6272(13)60088-6] [PMID: 23427406]
[39]
Andleeb S, Ejaz M, Awan UA, et al. In vitro screening of mucus and solvent extracts of Eisenia foetida against human bacterial and fungal pathogens. Pak J Pharm Sci 2016; 29(3): 969-77.
[PMID: 27166541]