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Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry

Author(s): Mohammed Yosri*, Mahmoud M. Elaasser, Marwa M. Abdel-Aziz, Hanna Y. Ahmed and Basma H. Amin

DOI: 10.2174/1871523021666220211110136

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Evaluation of Pleotropic Protective Activity of Capparis spinose Extract on Arthritis Rat Model

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Abstract

Background: Capparis spinosa grows in Asian and Mediterranean desert areas. Different parts of Capparis spinosa, including flowers, have been used in various folk medicine applications.

Objective: This study aims to evaluate the anti-arthritic potential of ethanolic extract of Egyptian Capparis spinosa flowers in a rat model of rheumatoid arthritis. Moreover, analysis of Capparis spinosa extract was performed using LC-qTOF-MS/MS.

Methods: Animals were split into six groups: negative control group, induced arthritic animals, arthritic rats receiving 7, 14 and 28 mg/kg of Capparis spinosa extract, respectively, in three groups to detect the optimum dose, and the induced group receiving a standard drug. The arthritic score was checked daily for 15 days after induction. After animals were sacrificed, their joints and muscles were subjected to microscopic and ultra-structure examinations. Ex vivo culturing of osteoclasts was performed. Cytokine levels were measured in all examined groups.

Results: The results revealed 7 mg/kg of Capparis spinosa extract as the optimal dose, which decreased inflammation signs through controlling chondrocytes, osteoclasts, and levels of inflammatory mediators.

Conclusion: LC-Mass analysis revealed Capparis spinosa extract to contain a mixture of flavonol glycosides, flavan-3-ols and hydroxycinnamic acid derivatives, which may provide beneficial multifunction in regulating arthritic symptoms.

Keywords: Capparis spinosa, adjuvant induced arthritis rats, histology, osteoclasts, cytokines, rheumatoid arthritis (RA).

Graphical Abstract

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