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Current Neurovascular Research

Author(s): Rian Q. Landers-Ramos*, Katherine I. Kim, Brent Hickey, Frederick M. Ivey, Charlene E. Hafer-Macko, Richard F. Macko, Alice S. Ryan and Steven J. Prior

DOI: 10.2174/1567202618666210406125558

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CD31+ Circulating Angiogenic Cell Number and Subtypes are Reduced in Individuals with Chronic Stroke

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Abstract

Background and Purpose: Reduced number and function of CD31+ circulating angiogenic cells (CACs) may explain vascular complications associated with the chronic phase stroke. The purpose of this study was to quantify CD31+ CAC paracrine function, total number and number of various subtypes of CD31+ CACs in individuals with chronic stroke compared with controls.

Methods: Peripheral blood mononuclear cells were isolated from chronic stroke participants and controls. CD31+ cells were quantified by flow cytometry, as was co-expression of CD31 in combination with CD14, CD3, CD11b, or CD34. Immunomagnetically selected CD31+ cells were cultured, and conditioned medium was used in a capillary-like network assay.

Results: Significantly lower levels of CD31+ CACs were found in stroke participants compared with controls (-24%; P=0.04). Additionally, CD31+/CD14+, CD31+/CD11b+ and CD31+/CD3+ cells were significantly lower in the chronic stroke group compared with controls (-45%, P=0.02; -47%, P=0.02 and -32%, P=0.03, respectively). There was no group effect on CD31+ CAC conditioned media-mediated capillary-like network formation.

Conclusion: CD31+ CACs and subtypes may serve as potential therapeutic targets in chronic stroke recovery.

Keywords: Circulating angiogenic cells, CD31, chronic stroke, angiogenic T cell, paracrine, angiogenesis.

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