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Current Analytical Chemistry

Author(s): Lijun Yan, Bo Shao, Xiaoping Zhang*, Yanyan Niu, Wendi Dang, Hui Cheng, Guangjiu Li and Wei Sun*

DOI: 10.2174/1573411016999200820160754

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Development of an Electrochemical DNA Biosensor Based on Gold Nanoparticles and Thiol Graphene Nanocomposite for Detection of a Specific nuc Gene from Staphylococcus aureus

Page: [418 - 425] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: An electrochemical DNA biosensor based on gold nanoparticles (AuNPs) and thiol graphene (TGR) nanocomposite modified carbon ionic liquid electrode (CILE) was developed to detect a specific nuc gene of Staphylococcus aureus, which was further used in the analysis of PCR amplification samples from unfrozen pork.

Objective: The development of a DNA biosensor derived from AuNPs-coated TGR could be used as a novel sensing method for the detection of specific ssDNA sequences in biological and clinical samples.

Methods: The electrochemical properties of modified CILE electrodes were determined by cyclic voltammetry and electrochemical impedance spectroscopy, and the electrochemical performances of the biosensor were investigated by differential pulse voltammetry.

Results: This gene sensor was able to detect the specific nuc gene from Staphylococcus aureus over the concentration range from 1.0×10-15 mol L-1 to 1.0×10-6 mol L-1 with a limit of detection of 4.5×10-16 mol L-1 (3σ), and it was applied in the detection of Staphylococcus aureus in an unfrozen pork sample after PCR amplification of the nuc gene with satisfactory results.

Conclusion: This gene biosensor showed high sensitivity and good selectivity, wide detection range and low detection limit, which demonstrated an effective tool to detect specific nuc gene sequences of Staphylococcus aureus.

Keywords: Gold nanoparticles, thiol graphene, specific nuc gene of Staphylococcus aureus, electrochemical DNA biosensor, ionic liquid, carbon ionic liquid electrode.

Graphical Abstract

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