Generic placeholder image

Current Enzyme Inhibition

Author(s): Edhem Hasković, Safija Herenda*, Zehra Halilović, Snežana Unčanin, Denis Hasković and Ena Deljkić

DOI: 10.2174/1573408016999200817173859

DownloadDownload PDF Flyer Cite As
Investigation of the Effect of Loratadine and Calcium Ions on Oxidoreductase Activity of Catalase Enzyme

Page: [26 - 32] Pages: 7

  • * (Excluding Mailing and Handling)

Abstract

Background: The Spectrophotometric method is one of the most suitable analytical techniques for testing the activity of enzymes under the influence of various factors.

Methods: The effect of H1-antihistamines of loratadine and calcium ions on enzyme catalase under in vitro conditions was investigated in this paper.

Results and Discussion: It has been shown that loratadine is a partial inhibitor of catalase, but this effect is diminished in the presence of calcium ions. Calcium as well as other cations are important for many biological and cellular functions. The kidneys play a central role in the homeostasis of these ions. The activity of the catalase enzyme under the given conditions, the type of inhibition, and the kinetic parameters of the enzyme reaction were determined.

Conclusion: We concluded that loratadine is a partially competitive inhibitor.

Keywords: Calcium ions, catalase, enzyme inhibition, inhibitor, loratadine, spectrophotometric method.

Graphical Abstract

[1]
Rang HP, Dale MM, Ritter JM, Moore PK. Farmakologija Beograd 2005; 23: 229-31.
[2]
Rashtbari S, Dehghan G, Yekta R, Jouyban A. Investigation of the binding mechanism and inhibition of bovine liver catalase by quercetin: Multi-spectroscopic and computational study. Bioimpacts 2017; 7(3): 147-53.
[http://dx.doi.org/10.15171/bi.2017.18] [PMID: 29159142]
[3]
Pavlov N. Racionalna primjena antihistaminika u alergologiji. Paediatria Croatica. Supplement 2012; 56: 128-34.
[4]
Friedman PA, Gesek FA. Cellular calcium transport in renal epithelia: measurement, mechanisms, and regulation. Physiol Rev 1995; 75(3): 429-71.
[http://dx.doi.org/10.1152/physrev.1995.75.3.429] [PMID: 7624390]
[5]
Cerletti A, Rothlin E. The pharmacological basis of calcium-antihistamine combination. Int Arch Allergy Appl Immunol 1955; 6(4-6): 230-42.
[http://dx.doi.org/10.1159/000228180] [PMID: 14391928]
[6]
Houillier P. Calcium-sensing in the kidney. Curr Opin Nephrol Hypertens 2013; 22(5): 566-71.
[PMID: 23917029]
[7]
Blaine J, Chonchol M, Levi M. Renal control of calcium, phosphate, and magnesium homeostasis. Clin J Am Soc Nephrol 2015; 10(7): 1257-72.
[http://dx.doi.org/10.2215/CJN.09750913] [PMID: 25287933]
[8]
Suzuki Y, Burzle M, Hediger MA. Physiology and patology of calcium and magnesium transport.Chapter 22 in Klaus Olgaard (ed) The spectrum of mineral and bone disorders in chronic kidney disease. Oxford: University press 2010; pp. 369-77.
[9]
Johnson JA, Kumar R. Renal and intestinal calcium transport: roles of vitamin D and vitamin D-dependent calcium binding proteins. Semin Nephrol 1994; 14(2): 119-28.
[PMID: 8177979]
[10]
Felsenfeld A, Rodriguez M, Levine B. New insights in regulation of calcium homeostasis. Curr Opin Nephrol Hypertens 2013; 22(4): 371-6.
[http://dx.doi.org/10.1097/MNH.0b013e328362141e PMID: 23736839]
[11]
Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991; 196(2-3): 143-51.
[http://dx.doi.org/10.1016/0009-8981(91)90067-M] [PMID: 2029780]
[12]
Assanasen P, Naclerio RM. Antiallergic anti-inflammatory effects of H1-antihistamines in humans. Clin Allergy Immunol 2002; 17: 101-39.
[PMID: 12113215]
[13]
Fontes R, Ribeiro JM, Sillero A. Inhibition and activation of enzymes. The effect of a modifier on the reaction rate and on kinetic parameters. Acta Biochim Pol 2000; 47(1): 233-57.
[http://dx.doi.org/10.18388/abp.2000_4082] [PMID: 10961698]
[14]
Strelow JI, Dewe W, Iversen PW, Brooks HB, Radding JA. Assay Guidance Manual Mechanism of Action Assays for Enzymes. Bethesda, MD: Eli Lilly & Company and the National Center for Advancing Translational Sciences 2004; p. 58.
[15]
Perutz M. Mechanisms of Cooperativity and Allosteric Regulation in Proteins. New York: Cambridge University Press 1990; p. 50.
[16]
Spence RA, Kati WM, Anderson KS, Johnson KA. Mechanism of inhibition of HIV-1 reverse transcriptase by nonnucleoside inhibitors. Science 1995; 267(5200): 988-93.
[http://dx.doi.org/10.1126/science.7532321] [PMID: 7532321]
[17]
Navia MA, Murcko MA. Use of structural information in drug design. Curr Opin Struct Biol 1992; 2: 202-10.
[http://dx.doi.org/10.1016/0959-440X(92)90147-Y]
[18]
Nezami A, Kimura T, Hidaka K, et al. High-affinity inhibition of a family of Plasmodium falciparum proteases by a designed adaptive inhibitor. Biochemistry 2003; 42(28): 8459-64.
[http://dx.doi.org/10.1021/bi034131z] [PMID: 12859191]
[19]
Copeland RA. Evaluation of Enzyme Inhibitors in Drug DiscoveryMethods of biochemical analysis A guide for Medicinal Chemists and Pharmacologists. New Jersey: Wiley-Interscience 2005.
[20]
Maragoni AG Enzyme Kinetics. A Modern Aproach. New Jersey: Wiley-Interscience 2003; pp. 61-2.
[21]
Letari O, Miozzo A, Folco G, et al. Effects of loratadine on cytosolic Ca2+ levels and leukotriene release: novel mechanisms of action independent of the anti-histamine activity. Eur J Pharmacol 1994; 266(3): 219-27.
[http://dx.doi.org/10.1016/0922-4106(94)90130-9] [PMID: 8174605]
[22]
Berthon B, Taudou G, Combettes L, et al. In vitro inhibition, by loratadine and descarboxyethoxyloratadine, of histamine release from human basophils, and of histamine release and intracellular calcium fluxes in rat basophilic leukemia cells (RBL-2H3). Biochem Pharmacol 1994; 47(5): 789-94.
[http://dx.doi.org/10.1016/0006-2952(94)90478-2] [PMID: 7510965]
[23]
Matysiak K, Matuszewski M, Feleszko W. Calcium preparations do not inhibit allergic reactions: a randomized controlled trial. Polish Articles of Internal Medicine 2017; 127(9): 582-8.
[PMID: 28724883]
[24]
Riccardi D, Brown EM. Physiology and pathophysiology of the calcium-sensing receptor in the kidney. Am J Physiol Renal Physiol 2010; 298(3): F485-99.
[http://dx.doi.org/10.1152/ajprenal.00608.2009] [PMID: 19923405]
[25]
Beaven MA, Rogers J, Moore JP, Hesketh TR, Smith GA, Metcalfe JC. The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. J Biol Chem 1984; 259(11): 7129-36.
[PMID: 6202691]
[26]
Bisswanger H. Enzyme kinetics. Principles and methods. Weinheim: Wiley-VCH Verlag GmbH & Co. KGAa 2008; 117-118: 182.