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Current Chromatography

Author(s): Samia Sams, Mohammad Khairul Alam*, Monira Ahsan and Sheikh Nazrul Islam

DOI: 10.2174/2213240607999200731010929

Cite As
Total Carotenoids Content and Assessment of Carotene Profile by High- Performance Liquid Chromatography in Selected Vegetables of Bangladesh with Special Reference to Some Unconventional Varieties

Page: [24 - 31] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: Carotenoids are natural plant pigments and precursors of vitamin A, which provide a health benefit, protect against chronic degenerative diseases, and contribute to immune functions. Utilization and identification of foods with a high content of carotenoids received greater attention nowadays.

Objective: The present study was aimed at evaluating total carotenoids content (TCC) and carotene composition of unconventional leafy vegetables growing in Bangladesh.

Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Acetone–petroleum ether extraction followed by spectrophotometric measurement was utilized for quantification of TCC. High-performance liquid chromatography (HPLC- DAD) was used to separate and quantify carotene components.

Results and Discussion: The TCC values ranged from 129.38 μg/100 g edible portion (EP) in Helencha to 12803.31 μg/100 g EP in Roktodrone. The study findings also revealed that the selected plant samples exhibited high content of β-carotene ranging from 120.99 μg/100 g EP in Parul to 11301.16 μg/100 g EP in Roktodrone. It was also observed that most of the vegetables analyzed did not have α-carotene, lycopene, β-cryptoxanthin or it was in such a small quantity that could not be detected with HPLC.

Conclusion: It can be suggested that regular intake of these vegetables may provide pro-vitamin A. In order to address the vitamin A deficiency and to maintain biodiversity, the analysis of carotenoids composition of these unconventional plant foods is to be initiated and make it available to the mass population. It would also fill up the data gap in the existing food composition table of Bangladesh.

Keywords: Vitamin A, β-carotene, carotenoids, carotene profile, HPLC-DAD, unconventional vegetables.

[1]
Alam, M.K.; Rana, Z.H.; Islam, S.N.; Akhtaruzzaman, M. Comparative assessment of nutritional composition, polyphenol profile, antidiabetic and antioxidative properties of selected edible wild plant species of Bangladesh. Food Chem., 2020, 320126646
[http://dx.doi.org/10.1016/j.foodchem.2020.126646] [PMID: 32229398]
[2]
Rana, Z.H.; Alam, M.K.; Akhtaruzzaman, M. Nutritional Composition, Total Phenolic Content, Antioxidant and α-Amylase Inhibitory Activities of Different Fractions of Selected Wild Edible Plants. Antioxidants, 2019, 8(7), 203.
[http://dx.doi.org/10.3390/antiox8070203] [PMID: 31266183]
[3]
Alam, M.K.; Rana, Z.H.; Islam, S.N.; Akhtaruzzaman, M. Total phenolic content and antioxidant activity of methanolic extract of selected wWild leafy vegetables grown in bangladesh: A cheapest source of antioxidants. Potravin. Slovak J. Food Sci., 2019, 13(1), 287-293.
[http://dx.doi.org/10.5219/1107]
[4]
Alam, M.K.; Rana, Z.H.; Islam, S.N. Comparison of the proximate composition, total carotenoids and total polyphenol content of nine orange-fleshed sweet potato varieties grown in Bangladesh. Foods, 2016, 5(3), 64.
[http://dx.doi.org/10.3390/foods5030064] [PMID: 28231159]
[5]
Alam, M.K.; Rana, Z.H.; Kabir, N.; Begum, P.; Kawsar, M.; Khatun, M.; Ahsan, M.; Islam, S.N. Total Phenolics, Total Carotenoids and Antioxidant Activity of Selected Unconventional Vegetables Growing in Bangladesh. Curr. Nutr. Food Sci., 2019, 15
[http://dx.doi.org/10.2174/1573401315666191209095515]
[6]
Johnson, E.J. The role of carotenoids in human health. Nutr. Clin. Care, 2002, 5(2), 56-65.
[http://dx.doi.org/10.1046/j.1523-5408.2002.00004.x] [PMID: 12134711]
[7]
Hercberg, S.; Kesse-Guyot, E.; Druesne-Pecollo, N.; Touvier, M.; Favier, A.; Latino-Martel, P.; Briançon, S.; Galan, P. Incidence of cancers, ischemic cardiovascular diseases and mortality during 5-year follow-up after stopping antioxidant vitamins and minerals supplements: a postintervention follow-up in the SU.VI.MAX Study. Int. J. Cancer, 2010, 127(8), 1875-1881.
[http://dx.doi.org/10.1002/ijc.25201] [PMID: 20104528]
[8]
Abdel-Aal, S.M.; Akhtar, H.; Zaheer, K.; Ali, R. Dietary sources of lutein and zeaxanthin carotenoids and their role in eye health. Nutrients, 2013, 5(4), 1169-1185.
[http://dx.doi.org/10.3390/nu5041169] [PMID: 23571649]
[9]
Yaacob, J.S.; Abdul Halim, N.A.; Ashokhan, S.; Ali, H.; Othman, R. Distribution of carotenoids and vitamin a activity in aerial organs of selected underutilized malaysian “ulam” or traditional vegetables (averrhoa carambola, manihot esculenta and ipomoea batatas). Pigm. Resin Technol., 2019, 48(2), 148-155.
[http://dx.doi.org/10.1108/PRT-02-2018-0019]
[10]
Islam, S.N.; Nusrat, T.; Begum, P.; Ahsan, M. Carotenoids and β- carotene in orange fleshed sweet potato: A possible solution to vitamin A deficiency. Food Chem., 2016, 199, 628-631.
[http://dx.doi.org/10.1016/j.foodchem.2015.12.057] [PMID: 26776017]
[11]
Ghani, A. Medicinal Plants of Bangladesh: Chemical Constituents and Uses; Dhaka Asiat. Soc.: Bangladesh, 2003.
[12]
Sarker, U.; Hossain, M.M.; Oba, S. Nutritional and antioxidant components and antioxidant capacity in green morph Amaranthus leafy vegetable. Sci. Rep., 2020, 10(1), 1336.
[http://dx.doi.org/10.1038/s41598-020-57687-3] [PMID: 31992722]
[13]
Sarker, U.; Oba, S. Protein, dietary fiber, minerals, antioxidant pigments and phytochemicals, and antioxidant activity in selected red morph Amaranthus leafy vegetable. PLoS One, 2019, 14(12)e0222517
[http://dx.doi.org/10.1371/journal.pone.0222517] [PMID: 31830064]
[14]
Southgate, D.A.T.; Greenfield, H. Guidelines for the production, management and use of food composition data: An in foods project. Food Sci. Nutr., 2017, 42(1), 15-23.
[http://dx.doi.org/10.1080/09543465.1988.11904124]
[15]
Gupta, S.; Jyothi Lakshmi, A.; Manjunath, M.N.; Prakash, J. Analysis of nutrient and antinutrient content of underutilized green leafy vegetables. Lebensm. Wiss. Technol., 2005, 38(4), 339-345.
[http://dx.doi.org/10.1016/j.lwt.2004.06.012]
[16]
Raju, M.; Varakumar, S.; Lakshminarayana, R.; Krishnakantha, T.P.; Baskaran, V. Carotenoid composition and vitamin A activity of medicinally important green leafy vegetables. Food Chem., 2007, 101(4), 1598-1605.
[http://dx.doi.org/10.1016/j.foodchem.2006.04.015]
[17]
Lakshminarayana, R.; Raju, M.; Krishnakantha, T.P.; Baskaran, V. Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography. J. Agric. Food Chem., 2005, 53(8), 2838-2842.
[http://dx.doi.org/10.1021/jf0481711] [PMID: 15826027]
[18]
Rajyalakshmi, P.; Venkatalaxmi, K.; Venkatalakshmamma, K.; Jyothsna, Y.; Devi, K.B.; Suneetha, V. Total carotenoid and beta- carotene contents of forest green leafy vegetables consumed by tribals of south India. Plant Foods Hum. Nutr., 2001, 56(3), 225-238.
[http://dx.doi.org/10.1023/A:1011125232097] [PMID: 11442223]
[19]
Steiner-Asiedu, M.; Agbemafle, I.; Setorglo, J.; Danquah, A.O.; Anderson, A.K. Carotenoids content of corchorus olitorius and solanum macrocarpon - commonly used ghanaian vegetables. Int. Food Res. J., 2014, 21(5), 2063-2067.
[20]
Mageney, V.; Baldermann, S.; Albach, D.C. Intraspecific variation in carotenoids of brassica oleracea var. sabellica. J. Agric. Food Chem., 2016, 64(16), 3251-3257.
[http://dx.doi.org/10.1021/acs.jafc.6b00268] [PMID: 27045759]
[21]
Aizawa, K.; Inakuma, T. Quantitation of carotenoids in commonly consumed vegetables in Japan. Food Sci. Technol. Res., 2007, 13(3), 247-252.
[http://dx.doi.org/10.3136/fstr.13.247]
[22]
Neugart, S.; Baldermann, S.; Ngwene, B.; Wesonga, J.; Schreiner, M. Indigenous leafy vegetables of Eastern Africa - A source of extraordinary secondary plant metabolites. Food Res. Int., 2017, 100(Pt 3), 411-422.
[http://dx.doi.org/10.1016/j.foodres.2017.02.014] [PMID: 28964364]
[23]
Burri, B.J.; La Frano, M.R.; Zhu, C. Absorption, metabolism, and functions of β-cryptoxanthin. Nutr. Rev., 2016, 74(2), 69-82.
[http://dx.doi.org/10.1093/nutrit/nuv064] [PMID: 26747887]