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Current Genomics

Author(s): Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong*

DOI: 10.2174/1389202921999200716103758

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Discrepant mRNA and Protein Expression in Immune Cells

Page: [560 - 563] Pages: 4

  • * (Excluding Mailing and Handling)

Abstract

With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expression will enhance the precision and credibility of the results.

Keywords: Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.

Graphical Abstract

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