Generic placeholder image

Current Chromatography

Author(s): Nesrine T. Lamie* and Hany H. Monir

DOI: 10.2174/2213240607999200611154615

Cite As
Diode Array Detection for UPLC Determination of Cinnarizine and Dimenhydrinate in their Combined Dosage Form

Page: [101 - 105] Pages: 5

  • * (Excluding Mailing and Handling)

Abstract

Background: Cinnarizine is used to treat nausea and vomiting accompanied by motion sickness. Dimenhydrinate is used in the treatment of nausea and vomiting and dizziness. The coformulation of the two drugs showed the lowest rate of adverse effects compared to single dimenhydrinate.

Objective: A fully validated ultra-performance liquid chromatographic method has been conducted for the simultaneous estimation of cinnarizine (CIN) and dimenhydrinate (DIM).

Materials and Methods: The UPLC method used Acquity Column as stationary phase and mobile phase methanol: buffer (pH = 3.5 ± 0.05) and acetonitrile in the ratio of 50: 25: 25 at a flow rate of 0.2 mL/min. Detection was performed by DAD at 260 nm.

Results and Discussion: Retention time was 0.71 and 1.12 min for DIM and CIN, respectively. The linearity was found to be 1-40 μg/mL and 2-80 μg/mL for CIN and DIM; respectively.

Conclusion: The method was appropriately used for the quantitation of both drugs in pure form, synthetic mixtures and tablet preparation.

Keywords: Cinnarizine, dimenhydrinate, diode array detector, UPLC, retention time, nausea.

[1]
Budavari S. The Merck Index: An Encyclopedia of Chemicals, Drugs and Biologicals 14th ed Merck & Co, Inc Whitehouse Station, NJ, USA. 2006.
[2]
Sweetman SC. Martindale-The Complete Drug Reference 37th ed The Pharmaceutical Press London, UK. 2011.
[3]
British Pharmacopoeia. Stationary Office, Medicines and healthcare products regulatory agency, London, 2010. pp. 507,703-508,704
[4]
Shah BP, Patel UP. First derivative spectrophotometric method for the simultaneous estimation of cinnarizine and dimenhydrinate in their combined dosage form. Int J Univ Pharm Bio Sci 2012; 2(2): 238-47.
[5]
Shah BP, Patel UP. Q-Absorbance ratio spectrophotometric method for the simultaneous estimation of cinnarizine and dimenhydrinate in their combined dosage form. J Pharma Sci Res 2012; 2(2): 83-7.
[6]
Lamie N. Simultaneous determination of cinnarizine and dimenhydrinate in binary mixture using, first derivative of ratio spectra and bivariate spectrophotometric techniques. Analyt Chem Indian J 2014; 14(3): 93-9.
[7]
Lamie NT. Comparative study of spectrophotometric methods manipulating ratio spectra: an application on pharmaceutical binary mixture of cinnarizine and dimenhydrinate. Spectrochim Acta A Mol Biomol Spectrosc 2015; 141: 193-201.
[http://dx.doi.org/10.1016/j.saa.2015.01.033] [PMID: 25677532]
[8]
Lamie NA. Yehia. Development of normalized spectra manipulating spectrophotometric methods for simultaneous determination of Dimenhydrinate and Cinnarizine binary mixture. Spectrochim Acta A Mol Biomol Spectrosc 2015; 150: 142-50.
[http://dx.doi.org/10.1016/j.saa.2015.05.048] [PMID: 26037499]
[9]
Sayed N. Abdelrahman, M. Salama, F. Badawy, A.l. Determination of dimenhydrinate and cinnarizine in combined dosage form in presence of cinnarizine impurity. Eur J Chem 2015; 6(4): 475-81.
[10]
Tawakkol M. El-Zeiny, M. Hemdan. Full spectrum and selected spectrum based chemometric methods for the simultaneous determination of Cinnarizine and Dimenhydrinate in laboratory prepared mixtures and pharmaceutical dosage form. Spectrochim Acta A Mol Biomol Spectrosc 2017; 173: 892-6.
[http://dx.doi.org/10.1016/j.saa.2016.10.055] [PMID: 27837737]
[11]
Patel PA, Kadikar KH, Shah RR, Patel PD, Tank KP. Simultaneous determination of cinnarizine and dimenhydrinate in binary mixture using chromatographic methods. Pharma Sci Monitor 2012; 54(1): 2586-600.
[12]
Lamie NT, Monir HH. Simultaneous determination of cinnarizine and dimenhydrinate in binary mixture using chromatographic methods. J Chromatogr Sci 2016; 54(1): 36-42.
[PMID: 26354946]
[13]
El-Kafrawy DSM, Belal TS. Validated HPTLC method for the simultaneous determination of cinnarizine and dimenhydrinate in their combined dosage form. J Assoc Arab Univ Basic ApplSci 2016; 19: 14-22.
[http://dx.doi.org/10.1016/j.jaubas.2014.06.004]]
[14]
Aboul-Enein HY, Ali I. Determination of tadalafil in pharmaceutical preparation by HPLC using monolithic silica column. Talanta 2005; 65(1): 276-80.
[http://dx.doi.org/10.1016/j.talanta.2004.06.012] [PMID: 18969796]
[15]
Ali I, Gaitonde VD, Aboul-Enein HY, Hussain A. Chiral separation of beta-adrenergic blockers on CelluCoat column by HPLC. Talanta 2009; 78(2): 458-63.
[http://dx.doi.org/10.1016/j.talanta.2008.11.043] [PMID: 19203609]
[16]
Ali I, Aboul-Enein HY. Enantioseparation of some clinicall used drugs by HPLC using cellulose Tris (3,5 dichlorophenylcarbamate) chiral stationary phase. Biomed. Chromatogr., 2003; 17(2,3): 113-7.
[17]
Aboul-Enein HY, Ali I J. Comparative study of the enantiomeric resolution of chiral antifungal drugs econazole, miconazole and sulconazole by HPLC on various cellulose chiral columns in normal phase mode Pharm Biomed Analysis 2002; 27(3, 4): 441-6.
[18]
Ali I, Aboul-Enein HY. Impact of immobilized polysaccharide chiral stationary phases on enantiomeric separations. J Sep Sci 2006; 29(6): 762-9.
[http://dx.doi.org/10.1002/jssc.200500372]
[19]
International Conference on Harmonization (ICH), Q2B: Validation of Analytical Procedures: Methodology. US FDA Federal Register, 1997.