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Current Pharmaceutical Biotechnology

Author(s): Sonia Mann, Ankita Sharma, Ashish Sarkar, Rupsi Kharb, Rajesh Malhotra, Barun Datta, Rajinder K. Gupta and Sagarika Biswas*

DOI: 10.2174/1389201021666191210114127

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Evaluation of Anti-inflammatory Effects of Choerospondias axillaris Fruit’s Methanolic Extract in Synoviocytes and CIA Rat Model

Page: [596 - 604] Pages: 9

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Abstract

Background: Rheumatoid Arthritis (RA) is an autoimmune, systemic disease mainly affecting joints. Presently, there is no specific treatment/ drug available for curing RA except few supportive medicines. Therefore, the focus has been shifted to medicinal plants for the treatment of such diseases. Choerospondias axillaris commonly known as Lupsi/Lapsi and has been reported to have several properties for the treatment of various diseases.

Objective: The present study has been conducted to explore the anti-inflammatory effects of Choerospondias axillaris fruit extract on Synoviocytes (FLS) and Collagen-Induced Arthritis (CIA) rat model.

Methods: Methanolic extract of the Choerospondias axillaris fruit was used for determining phytochemical, antioxidant and anti-inflammatory properties. Antioxidant activity of Choerospondias axillaris fruit was determined by free radicals scavenging assays and bioactive compounds were identified via LC-MS/MS analysis. Anti-inflammatory effect was investigated in RA and Osteo Arthritis (OA) primary cells and also in Collagen Induced Arthritis (CIA) rat models. Further, the medicinal properties of anti-inflammatory bioactive compounds were supported by docking studies.

Results: In-vitro and in-vivo studies showed significant decrease in the levels of inflammatory cytokines. Docking analysis revealed that quercetin inhibits TNF-α having -9.1 kcal/mol binding energy and 10.13 μM inhibitory constant. Quercetin also inhibits IL-6 having -6.6 kcal/mol binding energy and 21.9 μM inhibitory constant.

Conclusion: Observed results suggest that the underutilized fruit Choerospondias axillaris can be used to reduce the inflammation of inflammatory diseases like RA.

Keywords: Choerospondias axillaris, phytochemicals, LC-MS/MS, anti-inflammatory, CIA, docking.

Graphical Abstract

[1]
Chitme, R.H.; Patel, P.N. Antiarthritis activity of Aristolochia bracteata extract in experimental animals. Open Natl. Pro. J., 2009, 2, 6-15.
[http://dx.doi.org/10.2174/1874848100902010006]
[2]
Bonini, S.A.; Premoli, M.; Tambaro, S.; Kumar, A.; Maccarinelli, G.; Memo, M.; Mastinu, A. Cannabis sativa: A comprehensive ethnopharmacological review of a medicinal plant with a long history. J. Ethnopharmacol., 2018, 227, 300-315.
[http://dx.doi.org/10.1016/j.jep.2018.09.004] [PMID: 30205181]
[3]
Mazumder, M.P.; Mondal, A.; Sasmal, D.; Arulmozhi, S.; Rathinavelusamy, P. Evaluation of antiarthritic and immunomodulatory activity of Barleria lupulina. Asian Pac. J. Trop. Biomed., 2012, 2, S1400-S1406.
[http://dx.doi.org/10.1016/S2221-1691(12)60425-0]
[4]
Kooti, W.; Moradi, M.; Akbari, S.A.; Sharafi-Ahvazi, N. AsadiSamani, M.; Ashtary-Larky, D. Therapeutic and pharmacological potential of Foeniculum vulgare Mill: A review. J. Herb. Med. Pharmacol., 2015, 4, 1-9.
[5]
Kumar, A.; Premoli, M.; Aria, F.; Bonini, S.A.; Maccarinelli, G.; Gianoncelli, A.; Memo, M.; Mastinu, A. Cannabimimetic plants: are they new cannabinoidergic modulators? Planta, 2019, 249(6), 1681-1694.
[http://dx.doi.org/10.1007/s00425-019-03138-x] [PMID: 30877436]
[6]
Kuruppu, A.I.; Paranagama, P.; Goonasekara, C.L. Medicinal plants commonly used against cancer in traditional medicine formulae in Sri Lanka. Saudi Pharm. J., 2019, 27(4), 565-573.
[http://dx.doi.org/10.1016/j.jsps.2019.02.004] [PMID: 31061626]
[7]
Rungratanawanich, W.; Cenini, G.; Mastinu, A.; Sylvester, M.; Wilkening, A.; Abate, G.; Bonini, S.A.; Aria, F.; Marziano, M.; Maccarinelli, G.; Memo, M.; Voos, W.; Uberti, D. γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice. Nutrients, 2019, 11(4), 753.
[http://dx.doi.org/10.3390/nu11040753] [PMID: 30935111]
[8]
Quang, C.M. Phytochemical and pharmacological evaluation of Choerospondias axillaris: A Vietnamese medicinal plant used to treat burns. Licentiate thesis 17, Monograph: Uppsala University, Sweden, 1994.
[9]
Nguyen, D.D.; Nguyen, N.H.; Nguyen, T.T.; Phan, T.S.; Nguyen, V.D.; Grabe, M.; Johansson, R.; Lindgren, G.; Stjernström, N.E.; Söderberg, T.A. The use of a water extract from the bark of Choerospondias axillaris in the treatment of second degree burns. Scand. J. Plast. Reconstr. Surg. Hand Surg., 1996, 30(2), 139-144.
[http://dx.doi.org/10.3109/02844319609056396] [PMID: 8815984]
[10]
Labh, S.N.; Shakya, S.R. Medicinal importance of Choerospondias axillaris (Roxb.) Burtt & Hill fruits in Nepal. Trop. Plant Res., 2016, 3, 463-469.
[11]
Li, Q.; Chen, J.; Li, T.; Liu, C.; Zhai, Y.; McClements, D.J.; Liu, J. Separation and characterization of polyphenolics from underutilized byproducts of fruit production (Choerospondias axillaris peels): inhibitory activity of proanthocyanidins against glycolysis enzymes. Food Funct., 2015, 6(12), 3693-3701.
[http://dx.doi.org/10.1039/C5FO00939A] [PMID: 26442714]
[12]
Diaz, P.; Jeong, S.C.; Lee, S.; Khoo, C.; Koyyalamudi, S.R. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chin. Med., 2012, 7(1), 26.
[http://dx.doi.org/10.1186/1749-8546-7-26] [PMID: 23176585]
[13]
Dawidowicz, A.L.; Olszowy, M. Mechanism change in estimating of antioxidant activity of phenolic compounds. Talanta, 2012, 97, 312-317.
[http://dx.doi.org/10.1016/j.talanta.2012.04.036] [PMID: 22841085]
[14]
Guo, Q.; Wang, Y.; Xu, D.; Nossent, J.; Pavlos, N.J.; Xu, J. Rheumatoid arthritis: pathological mechanisms and modern pharmacologic therapies. Bone Res., 2018, 6(6), 15.
[http://dx.doi.org/10.1038/s41413-018-0016-9] [PMID: 29736302]
[15]
Mun, S.; Lee, J.; Lim, M.K.; Lee, Y.R.; Ihm, C.; Lee, S.H.; Kang, H.G. Development of a novel diagnostic biomarker set for Rheumatoid arthritis using a proteomics approach. BioMed Res. Int., 2018, 20187490723
[http://dx.doi.org/10.1155/2018/7490723] [PMID: 30662913]
[16]
Scott, D.L.; Wolfe, F.; Huizinga, T.W. Rheumatoid arthritis. Lancet, 2010, 376(9746), 1094-1108.
[http://dx.doi.org/10.1016/S0140-6736(10)60826-4] [PMID: 20870100]
[17]
Caldwell, J.R. Efficacy and safety of diclofenac sodium in rheumatoid arthritis. Experience in the United States. Am. J. Med., 1986, 80(4B), 43-47.
[http://dx.doi.org/10.1016/0002-9343(86)90079-3] [PMID: 3518432]
[18]
Chan, F.K.; Lanas, A.; Scheiman, J.; Berger, M.F.; Nguyen, H.; Goldstein, J.L. Celecoxib versus omeprazole and diclofenac in patients with osteoarthritis and rheumatoid arthritis (CONDOR): a randomised trial. Lancet, 2010, 376(9736), 173-179.
[http://dx.doi.org/10.1016/S0140-6736(10)60673-3] [PMID: 20638563]
[19]
Biswas, S.; Sharma, S.; Saroha, A.; Bhakuni, D.S.; Malhotra, R.; Zahur, M.; Oellerich, M.; Das, H.R.; Asif, A.R. Identification of novel autoantigen in the synovial fluid of rheumatoid arthritis patients using an immunoproteomics approach. PLoS One, 2013, 8(2)e56246
[http://dx.doi.org/10.1371/journal.pone.0056246] [PMID: 23418544]
[20]
Koncić, M.Z.; Kremer, D.; Gruz, J.; Strnad, M.; Bisevac, G.; Kosalec, I.; Samec, D.; Piljac-Zegarac, J.; Karlović, K. Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions. Food Chem. Toxicol., 2010, 48(6), 1537-1542.
[http://dx.doi.org/10.1016/j.fct.2010.03.021] [PMID: 20304028]
[21]
Saeed, N.; Khan, M.R.; Shabbir, M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement. Altern. Med., 2012, 12, 221.
[http://dx.doi.org/10.1186/1472-6882-12-221] [PMID: 23153304]
[22]
Miliauskas, G.; Venskutonis, P.R.; Beek, T.A.V. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem., 2004, 85, 231-237.
[http://dx.doi.org/10.1016/j.foodchem.2003.05.007]
[23]
Mann, S.; Satpathy, G.; Gupta, R.K. Evaluation of nutritional and phytochemical profiling of Baccaurea ramiflora Lour. syn. Baccaurea sapida (Roxb.). Mull. Arg. Fruits. Ind. J. Trad. Know., 2016, 15, 135-142.
[24]
Gupta, D.; Gupta, R.K. Bioprotective properties of Dragon’s blood resin: in vitro evaluation of antioxidant activity and antimicrobial activity. BMC Complement. Altern. Med., 2011, 11, 13.
[http://dx.doi.org/10.1186/1472-6882-11-13] [PMID: 21329518]
[25]
Satpathy, G.; Tyagi, Y.; Gupta, R.K. Preliminary evaluation of nutraceutical and therapeutic potential of raw Spondias pinnata K., an exotic fruit of India. Food Res. Int., 2011, 44, 2076-2087.
[http://dx.doi.org/10.1016/j.foodres.2011.01.040]
[26]
Biswas, S.; Sahu, D.; Saroha, A.; Das, H.R. Glycoproteomic analysis of collagen induced arthritis in rats. Trends. In. Carbohydrate. Res., 2009, 1, 12-20.
[27]
Amri, O.; Zekhnini, A.; Bouhaimi, A.; Tahrouch, S.; Hatimi, A. Anti-inflammatory activity of methanolic extract from Pistacia atlantica Desf. leaves. Pharmacogn. J., 2018, 10, 71-76.
[http://dx.doi.org/10.5530/pj.2018.1.14]
[28]
Kołodziejska, J.; Kołodziejczyk, M. Diclofenac in the treatment of pain in patients with rheumatic diseases. Reumatologia, 2018, 56(3), 174-183.
[http://dx.doi.org/10.5114/reum.2018.76816] [PMID: 30042605]
[29]
Labh, S.N.; Shakya, S.R.; Kayasta, B.L. Extract of Medicinal lapsi Choerospondias axillaris (Roxb.) exhibit antioxidant activities during in vitro studies. J. Pharmacogn. Phytochem., 2015, 4, 194-197.
[30]
Chen, C. Sinapic acid and its derivatives as medicine in oxidative stress-induced diseases and aging. Oxid. Med. Cell. Longev., 2016, 20163571614
[http://dx.doi.org/10.1155/2016/3571614] [PMID: 27069529]
[31]
Guzman, J.D. Natural cinnamic acids, synthetic derivatives and hybrids with antimicrobial activity. Molecules, 2014, 19(12), 19292-19349.
[http://dx.doi.org/10.3390/molecules191219292] [PMID: 25429559]
[32]
Gallie, D.R. L-ascorbic Acid: a multifunctional molecule supporting plant growth and development. Scientifica (Cairo), 2013, 2013795964
[http://dx.doi.org/10.1155/2013/795964] [PMID: 24278786]
[33]
Randjelović, P.; Veljković, S.; Stojiljković, N.; Sokolović, D.; Ilić, I.; Laketić, D.; Randjelović, D.; Randjelović, N. The beneficial biological properties of salicylic acid. Acta Facultatis Medicae Naissensis UDC, 2015, 32(4), 259-265.
[http://dx.doi.org/10.1515/afmnai-2015-0026]
[34]
Lee, H.N.; Shin, S.A.; Choo, G.S.; Kim, H.J.; Park, Y.S.; Kim, B.S.; Kim, S.K.; Cho, S.D.; Nam, J.S.; Choi, C.S.; Che, J.H.; Park, B.K.; Jung, J.Y. Anti‑inflammatory effect of quercetin and galangin in LPS‑stimulated RAW264.7 macrophages and DNCB‑induced atopic dermatitis animal models. Int. J. Mol. Med., 2018, 41(2), 888-898.
[PMID: 29207037]
[35]
Koleva, I.I.; van Beek, T.A.; Linssen, J.P.; de Groot, A.; Evstatieva, L.N. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal., 2002, 13(1), 8-17.
[http://dx.doi.org/10.1002/pca.611] [PMID: 11899609]
[36]
Yuan, H.; Ma, Q.; Ye, L.; Piao, G. The traditional medicine and modern medicine from natural products. Molecules, 2016, 21(5), 559.
[http://dx.doi.org/10.3390/molecules21050559] [PMID: 27136524]
[37]
Zhang, X.; Guo, F. Natural antioxidants for health promotion and disease prevention. Front. Pharmacol., 2014, 5, 266.
[http://dx.doi.org/10.3389/fphar.2014.00266]
[38]
Pandey, K.B.; Rizvi, S.I. Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell. Longev., 2009, 2(5), 270-278.
[http://dx.doi.org/10.4161/oxim.2.5.9498] [PMID: 20716914]
[39]
Panche, A.N.; Diwan, A.D.; Chandra, S.R. Flavonoids: an overview. J. Nutr. Sci., 2016, 5e47
[http://dx.doi.org/10.1017/jns.2016.41] [PMID: 28620474]
[40]
Wink, M. Modes of action of herbal medicines and plant secondary metabolites. Medicines (Basel), 2015, 2(3), 251-286.
[http://dx.doi.org/10.3390/medicines2030251] [PMID: 28930211]
[41]
Wei, L.S.; Wee, W.; Siong, J.Y.; Syamsumir, D.F. Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract. Acta Med. Iran., 2011, 49(10), 670-674.
[PMID: 22071643]
[42]
Brewer, M.S. Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications. Compr. Rev. Food Sci. Food Saf., 2011, 10, 221-247.
[http://dx.doi.org/10.1111/j.1541-4337.2011.00156.x]
[43]
Manicourt, D.H.; Triki, R.; Fukuda, K.; Devogelaer, J.P.; Nagant de Deuxchaisnes, C.; Thonar, E.J. Levels of circulating tumor necrosis factor alpha and interleukin-6 in patients with rheumatoid arthritis. Relationship to serum levels of hyaluronan and antigenic keratan sulfate. Arthritis Rheum., 1993, 36(4), 490-499.
[http://dx.doi.org/10.1002/art.1780360409] [PMID: 8457224]
[44]
Hashizume, M.; Mihara, M. The roles of interleukin-6 in the pathogenesis of rheumatoid arthritis. Arthritis (Egypt), 2011, 2011765624
[http://dx.doi.org/10.1155/2011/765624] [PMID: 22046525]
[45]
Matsuno, H.; Yudoh, K.; Katayama, R.; Nakazawa, F.; Uzuki, M.; Sawai, T.; Yonezawa, T.; Saeki, Y.; Panayi, G.S.; Pitzalis, C.; Kimura, T. The role of TNF-alpha in the pathogenesis of inflammation and joint destruction in rheumatoid arthritis (RA): a study using a human RA/SCID mouse chimera. Rheumatology (Oxford), 2002, 41(3), 329-337.
[http://dx.doi.org/10.1093/rheumatology/41.3.329] [PMID: 11934972]
[46]
Shakiba, K.; Falcone, T. Tumour necrosis factor-alpha blockers: potential limitations in the management of advanced endometriosis? A case report. Hum. Reprod., 2006, 21(9), 2417-2420.
[http://dx.doi.org/10.1093/humrep/del179] [PMID: 16785259]
[47]
Siebert, S.; Tsoukas, A.; Robertson, J.; McInnes, I. Cytokines as therapeutic targets in rheumatoid arthritis and other inflammatory diseases. Pharmacol. Rev., 2015, 67(2), 280-309.
[http://dx.doi.org/10.1124/pr.114.009639] [PMID: 25697599]
[48]
Mann, S.; Sharma, A.; Biswas, S.; Gupta, R.K. Identification and molecular docking analysis of active ingredients with medicinal properties from edible Baccaurea sapida. Bioinformation, 2015, 11(9), 437-443.
[http://dx.doi.org/10.6026/97320630011437] [PMID: 26527853]