Abstract

Pathogenic bacteria constitute one of the most serious threats to human health. This has led to the development of technologies for the rapid detection of bacteria. Bio-nanogold-based lateral flow biosensors (LFBs) are a promising assay due to their low limit of detection, high sensitivity, good selectivity, robustness, low cost, and quick assay performance ability. The aim of this review is to provide a critical overview of the current variety of bio-nanogold LFBs and their targets, with a special focus on whole-cell and DNA detection of pathogenic bacteria. The challenges of bio-nanogold-based LFBs in improving their performance and accessibility are also comprehensively discussed.

Keywords: Bio-nanogold, Lateral flow biosensor, Pathogenic bacteria, Rapid detection, LFBs, Nitrocellular membrane.