Shaping and Cellular Uptake of Folic Acid Coated Gold and Magnetite Nanoparticles

Page: [166 - 172] Pages: 7

  • * (Excluding Mailing and Handling)

Abstract

Background: Gold and Iron Oxide nanoparticles NPs play as nanocarriers for a specific drug delivery and contrast agents. Intercellular uptake of these nanoparticles and targeting to individual cell and sub-cellular compartment is essential.

Objective: The aim of the current study is to evaluate the intracellular uptake of these NPs to specific tumor cells in vitro conjugated with folic acid with a goal of enhancing the efficiency of specific targeting to tumor cells.

Methods: We synthesized the nanoparticles by a chemical method and characterized by UV-Visible, FTIR, XRD, and TEM.

Results & Conclusion: The results revealed the conjugation of Gold and Iron Oxide nanoparticles with folic acid increased the intercellular uptake with high percent compared to non- conjugated nanoparticles.

Keywords: Gold, Iron oxide nanoparticles, folate receptors, intracellular uptake, folic acid, magnetite nanoparticles.

Graphical Abstract

[1]
Gautier J, Allard E, Monnier E, et al. Nanostructured Platforms for the Sustained and Local Delivery of Antibiotics in the Treatment of Osteomyelitis. J Control Release 2013; 169: 48-61.
[2]
Rana S, Bajaj A, Mout R, et al. Monolayer coated gold nanoparticles for delivery applications. Adv Drug Deliv Rev 2012; 64: 200-16.
[3]
Pustovalov V, Smetannikov A, Zharov V, et al. Photothermal and accompanied phenomena of selective nanophotothermolysis with gold nanoparticles and laser pulses. J Laser Phys Lett 2008; 5: 775.
[4]
Ferrari M. Cancer nanotechnology: opportunities and challenges. J Nat Rev 2005; 5: 161-71.
[5]
Zhou J, Caruntu D. Synthesis of porous magnetic hollow silica nanospheres for nanomedicine application. J Phys Chem 2007; 111: 17473-7.
[6]
Robinson I, Tung L, Maenosono S, et al. Synthesis of core-shell gold coated magnetic nanoparticles and their interaction with thiolated DNA. Nanoscale 2010; 2: 2624-30.
[7]
Heidari M, Astoria D J. Barara, An International Journal Devoted to Fundamental and Applied Research on Colloid and Interfacial Phenomena in Relation to Systems of Biological Origin. Colloids Surf B Biointerfaces 2013; 106: 117-25.8.
[8]
Lee N, Hyeon T. Designed synthesis of uniformly sized iron oxide nanoparticles for efficient magnetic resonance imaging contrast agents. J Chem Soc Rev 2012; 41: 2575-89.
[9]
Bu L, Xie J, Chen K, et al. Assessment and comparison of magnetic nanoparticles as MRI contrast agents in a rodent model of human hepatocellular carcinoma. J Contrast Media Mol Imaging 2012; 7: 363-72.
[10]
Lu Y, Philip S. Folate-mediated delivery of macromolecular anticancer therapeutic agents. J Low Advanced Drug Deliv Rev 2002; 54: 675-93.
[11]
Hwa S, Jeong J, Joe C. et al. Folate receptor mediated intracellular protein delivery using PLL–PEG–FOL conjugate. J Control Release 2005; 103: 625.
[12]
Corr S, Byrne S, Tekoriute R, et al. Linear Assemblies of Magnetic Nanoparticles as MRI Contrast Agents. J Am Chem Soc 2008; 130: 4214-5.
[13]
Yigit M, Moore A, Medarova Z. Magnetic nanoparticles for cancer diagnosis and therapy. J Pharm Res 2012; 29: 1180-8.
[14]
Tietze R, Lyer S, Durr S, et al. Nanoparticles for cancer therapy using magnetic forces. J Nanomedicine 2012; 7: 447-57.
[15]
Li C, Li L, Keate A. Targeting cancer gene therapy with magnetic nanoparticles. J Oncotarget 2012; 3: 365-70.
[16]
Turkevich J, Stevenson P, Hillier P. A Study of the nucleation and growth processes in the synthesis of colloidal gold. J Faraday Soc 1951; 11: 55.
[17]
Elsherbini A, Saber M, Aggag M, El-Shahawy A, Shokier H. Magnetic nanoparticle-induced hyperthermia treatment under magnetic resonance imaging. J Magn Reson Imaging 2011; 29(2): 272-80.
[18]
Henglein A, Meisel D. Radiolytic control of the size of colloidal gold nanoparticles. J Langmuir 1998; 14: 7392.
[19]
Kamat P. J. Phys. Photophysical, photochemical and photocatalytic aspects of metal nanoparticles. J Chem Biol 2002; 106: 7729.
[20]
Irshad A, Ahmad T. Size and shape dependant antifungal activity of gold nanoparticles: A case study of Candida. Colloids Surf B Biointerfaces 2013; 101: 162-70.
[21]
Vora A, Riga A, Dollimore D. Thermal stability of folic acid in the solid-state. J Thermochimica Acta 2002; 392-393: 209-20.
[22]
Haining C, Jiang H, Deng L, Gao X. Fabrication of cyclodextrin-functionalized superparamagnetic Fe3O4/amino-silane core–shell nanoparticles via layer-by-layer method. J Appl Surf Sci 2009; 255: 7974-80.
[23]
Sun C, Sze R, Zhang M. Folic acid‐PEG conjugated superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI. J Biomed Mater Res Part A 2006; 45: 550-7.
[24]
Sorkin A, Manojkumar A. Vesicle trafficking in cancer. New York: Springer 2013.