Abstract

Background: The purpose of the present study was the distribution of bla_OXA-1, bla_PER-1 and bla_VEB genes and the genotyping of these genes in extended-spectrum β-lactamases (ESBL) producing uropathogenic and diarrheagenic Escherichia coli isolates. Methods: Of 432 isolates, 58 uropathogenic E. coli (UPEC) and 56 diarrheagenic E. coli (DEC) isolates were shown to produce the ESBLs. The isolates were screened to present ESBLs genes by PCR. Pulsed-Field Gel Electrophoresis (PFGE) was used to genotype E. coli strains which possess ESBLs genes. Results: The bla_OXA-1 gene was detected in 17.24% of UPEC isolates and 1.78% of DEC isolates. Of all the isolates studied, none were positive for the bla_PER-1 and bla_VEB genes. PFGE revealed that the E. coli isolates possessed bla_OXA-1 gene comprised three distinct genotypes. Conclusions: The current study illustrated high rates of ESBL-resistant phenotypes of E. coli isolates from urine and diarrhea samples in Iran. The bla_OXA-1, bla_PER-1 and bla_VEB genes were found at low frequencies in studied isolates.

Keywords: Escherichia coli, extended-spectrum β-lactamases, genotyping, PFGE.

Graphical Abstract