Abstract

Optically pure secondary alcohols were efficiently prepared via a two-step, one-pot process involving a chemical oxidative agent in combination with an enantioselective ketone reducase Rhodotorula sp. AS2.2241 in tandem. The reaction proceeded successfully with 88∼99.5% enantiomeric excess in moderate to good conversions. A variety of substrates, including aromatic and aliphatic alcohols, could be tolerated.

Keywords: Deracemization, oxidative agent, Rhodotorula sp. AS2.2241, chemo-enzymatic, one-pot, secondary alcohols