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The Natural Products Journal

Author(s): Yuan Yuan Wu, Ling Ling, Anggelima, Du Lan, Tergel, Ya Nan Gao, Wen Cheng Cai, Shan Tong and Huan Wang*

DOI: 10.2174/0122103155261774230920033831

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Proteome-wide Profiling Reveals Molecular Mechanism Underlying the Therapeutic Effect of Mongolian Medicine Sulongga-4 on Pyloric Ligation-induced Gastroduodenal Ulcer in Rats

Article ID: e250923221344 Pages: 14

  • * (Excluding Mailing and Handling)

Abstract

Background: Gastroduodenal ulcer is one of the common global gastrointestinal diseases. Sulongga-4 is a traditional Mongolian medicine used for the treatment of gastroduodenal ulcer. However, the molecular mechanism underlying the therapeutic effect of Sulongga-4 on gastroduodenal ulcer in the proteome has not been clarified.

Objective: The purpose of this study was to explore the molecular mechanism behind the therapeutic efficacy of traditional Mongolian medicine Sulongga-4 on pyloric ligation-induced gastroduodenal ulcer in rats.

Methods: Gastroduodenal ulcer was induced by pyloric ligation in rats. The pathological changes of gastric and duodenal tissues were observed first, and then the serum levels of AST and ALT were measured. The significantly different proteins were verified by western blot and qRT-PCR.

Results: The proteomics results showed that the Mongolian medicine Sulongga-4 might act on pyloric ligation-induced gastroduodenal ulcer through differentially expressing several proteins, including RPL35, RPL37, and LOC102548628 in gastric tissue, as well as Serpin b1a, Serpin b6a, and Vtn in duodenal tissue by regulating ribosome, alcoholism and amoebiasis, and complement and coagulation cascade pathways. In addition, the changes in serum AST and ALT levels in rats showed gastroduodenal ulcer to be associated with liver injury.

Conclusion: Sulongga-4 has shown a robust therapeutic effect against gastric duodenal ulcer. This therapeutic effect may be mainly associated with pathways of ribosome, alcoholism in gastric tissue, and amoebiasis, as well as complement and coagulation cascades in duodenal tissue.

Graphical Abstract

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