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Anti-Cancer Agents in Medicinal Chemistry

Author(s): Masoumeh Hajizadeh, Salar Hafez Ghoran, Hewa Jalal Azeez, Mohammad Ali Hosseinpour Feizi and Esmaeil Babaei*

DOI: 10.2174/1871520621666211015141928

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Apoptotic Effect of Gemini Curcumin on MDA-MB-468 Breast Cancer Cell Line

Page: [2181 - 2188] Pages: 8

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Abstract

Background: Gemini Curcumin (Gemini-Cur) is the latest nanoformulation of curcumin with a significant apoptotic effect on cancer cell lines.

Objective: This in vitro study aims to evaluate the apoptotic effects of Gemini-Cur toward MDA-MB-468 breast cancer cell lines and further the related mechanism of apoptosis.

Methods: The cytotoxicity of Gemini-Cur toward MDA-MB-468 cell lines was tested using MTT assay. Furthermore, the expression ratio of Bax/Bcl-2 was evaluated by qRT-PCR. Consequently, the protein expression of Bax/Bcl-2, survivin, and caspase-3 was measured using western blotting.

Results: Having treated MDA-MB-468 cell lines with Gemini-Cur, the IC50 values were found to be 44.44 and 31.63 μM at 24 and 48 h, respectively. Our findings showed that Gemini-Cur significantly suppresses cancer cell proliferation in a time- and dose-dependent manner. Furthermore, the data revealed that curcumin nanoformulation induces apoptosis in MDA-MB-468 cells through modulation of the expression of Bax, Bcl-2, Survivin, and Caspase-3.

Conclusion: The data of the current study propose that Gemini-Cur can be considered a promising candidate against triple-negative breast cancer.

Keywords: Apoptosis, breast cancer, Bax/Bcl-2 ratio, gemini curcumin, MDA-MB-468, survivin, caspase-3.

Graphical Abstract

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