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Current Pharmaceutical Analysis

Author(s): Fuman Cai, Yuwei Dong, Shaosheng Lou, Zeping Ma, Ting Wu, Liuqing Yang, Congcong Wen* and Chenxiang Wang*

DOI: 10.2174/1573412916999200624155618

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Determination of three Unsaturated Fatty Acids in Pressure Ulcer Rats Using A UPLC-MS/MS Method

Page: [1008 - 1015] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: The serum levels of Docosahexaenoic Acid (DHA), Eicosapentaenoic Acid (EPA) and Arachidonic Acid (AA) under the state of Pressure Ulcers (PUs) are still unclear.

Introduction: In order to investigate serum levels of DHA, EPA, and AA in PUs rats, an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed and validated.

Methods: Chromatographic separation of DHA, EPA, AA was carried out on a BEH C18 column and gradient elute consisted of 5 mM ammonium acetate-0.1% formic acid and acetonitrile. Subsequently, fifty rats were divided into five groups (n=10), four PU groups (A-D) underwent various pressure and release time protocols, with group E as the control. The concentrations of DHA, EPA, AA from five groups were determined by using a validated method.

Results: The results showed there was good linearity for DHA (327.3/283.4), EPA (301.2/257.0), and AA (303.1/258.9) within 0.05-6.4 μg/mL. In control group, the levels of DHA, AA and EPA were 1.16±0.68, 0.59±0.19 and 0.78±0.21 μg/mL. At the end of modeling, concentrations of DHA, EPA and AA were increased after long and persistent pressure (>8 h). Especially, the level of DHA was significantly higher (P<0.01) than that of control group.

Conclusion: A stable, reliable and accurate UPLC-MS/MS for determination of DHA, EPA, AA in blood was developed. Serum concentrations of DHA, EPA and AA were altered differently after long and persistent pressure (>8 h), and DHA is a remarkable one.

Keywords: UPLC-MS/MS, pressure ulcer, docosahexaenoic acid, eicosapentaenoic acid, arachidonic acid, rat.

Graphical Abstract

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