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Anti-Cancer Agents in Medicinal Chemistry

Author(s): Zhila Rajaee, Samideh Khoei*, Alireza Mahdavian, Sakine Shirvalilou*, Seied R. Mahdavi and Marzieh Ebrahimi

DOI: 10.2174/1871520620666191216113052

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Radio-thermo-sensitivity Induced by Gold Magnetic Nanoparticles in the Monolayer Culture of Human Prostate Carcinoma Cell Line DU145

Page: [315 - 324] Pages: 10

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Abstract

Background and Objective: Prostate cancer is the second cause of death in men worldwide. In this study, the cytotoxic effects of PLGA polymer-coated gold Magnetic Nanoparticles (MGNPs), as a novel treatment to enhance radiation and thermal sensitivity in the presence of hyperthermia (43°C) and electron beam, on DU145 prostate cancer cells were investigated.

Methods: Nanoparticles were characterized using TEM, DLS, XRD and SAED methods. MGNPs entrance into the cells was determined using Prussian blue staining and TEM. Furthermore, the cytotoxic effects of combinatorial treatment modalities were assessed by applying colony and sphere formation assay.

Results: Our results revealed that the decrease of colony and sphere numbers after combinatorial treatment of hyperthermia and radiation in the presence of nanoparticles was significantly higher than the other treatment groups (P<0.05). This treatment method proved that it has the capability of eliminating most of the DU145 cells (80-100%), and increased the value of the linear parameter (α) to 4.86 times.

Conclusion: According to the study, magnetic gold nanoparticles, in addition to having a high atomic number, can effectively transmit heat produced inside them to the adjacent regions under hyperthermia, which increases the effects of radio-thermosensitivity, respectively.

Keywords: Prostate cancer, gold magnetite nanoparticles, electron radiation, hyperthermia, sphere formation assay, LQ model.

Graphical Abstract

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